Sothern blotting
  • The technique was developed by E.M. Southern in 1975.
  • The Southern blot is used to detect the presence of a particular DNA fragment in a sample.
  • The DNA detected can be a single gene, or it can be part of a larger piece of DNA such as a viral genome.
Southern blotting Procedure Northern blotting
  • The technique was developed by Alwine and his colleagues in 1979
  • Technique for detecting specific RNAs separated by electrophoresis by hybridization to a labeled DNA probe.
 
Northern Blotting Procedure
Southern blotting
Northern blotting
Southern name of inventor Northern a misnomer
Separation of DNA Separation of RNA
Denaturation needed Denaturation  not needed
Nitrocellulose filter membrane Amino benzyloxymethyl filter paper Membrane
DNA-DNA  Hybridization RNA-DNA Hybridization

10 Comments

We Love to hear from U :) Leave us a Comment to improve this site
Thanks for Visiting.....

  1. Mate this is top-notch educational stuff.

    ReplyDelete
  2. I love this it was a great answer!

    ReplyDelete
  3. Love this site! wonderful !. Thanks for providing such good information in such wonderful and understanding way

    ReplyDelete
  4. Love this site! wonderful !. Thanks for providing such good information in such wonderful and understanding way.

    ReplyDelete
  5. it was really helpful..

    ReplyDelete
  6. Helpful, thanks :)

    ReplyDelete

Post a Comment

We Love to hear from U :) Leave us a Comment to improve this site
Thanks for Visiting.....

Previous Post Next Post