10 Differences between BAC and YAC Vector

10 Differences between BAC and YAC Vector

A vector is any DNA molecule that has the ability to replicate inside the host to which the desired gene has integrated for cloning.

Vectors include plasmids, bacteriophages, cosmids, BAC, YAC, yeast vectors, shuttle vectors etc.

YAC and BAC vectors are two genetically engineered artificial vectors designed to clone large DNA fragments. Now both these vectors are very much modified based on requirements. Let’s differentiate the basic YAC and BAC vector in this post.

YAC	BAC
YAC vector means Yeast Artificial Chromosome vectors.	BAC vector means Bacterial Artificial Chromosome Vectors.
Developed by Burke and Olson in 1987	Developed  Melsimon et al 1992
Can replicate inside yeast Used to clone DNA sequences in yeast cells	Can replicate inside bacteria used to clone DNA sequences in bacterial cells
Basic Components of YAC vector ARS (Autonomously Replicating Sequence)  – Ori; origin of replication for Yeast CEN (Centromeric) –yeast centromere for  movement of YAC vectors  to daughter cells 2 TEL (Telomeric) -Telomeric region for behaving as yeast chromosome on linearization AmpR– Selectable marker genes for selection of transformed cells BamHI and EcoRI restriction sites for inserting our gene of interest	Basic components of BAC vector(pBAC108L first developed BAC vector) OriS – ori for bacteria repE – initiation & assembly of replication  complex  parA and parB – movement of BAC to daughter cells Selectable marker –Chloramphenicol Resistance for selection of transformed cells T7 and SP6 – Promote transcription of insert gene
Its construction is based on regions of yeast chromosome like ARS	BAC is a DNA construct, based on functional fertility plasmid (or F-plasmid), of  E. coli.
Linear vector inside yeast cell with 2 telomeric ends  just functions as yeast chromosomes without disturbing original chromosomes	Circular vector inside bacterium
Clone DNA fragments larger than 100 kb and up to 3,000 kb	Insert size is 150–350 kb.
It is used for physical mapping and sequencing of genomes. YAC libraries of human and Caenorhabditis elegans and many genomes First used in Human genome project later shifted to BAC vectors due to stability issues with YAC	It is used for physical mapping and sequencing of genomes. Used in HGP and in physical genetic maps of including Mus musculus, Arabidopsis thaliana, and Homo sapiens.
Advantage of YAC: large insert size	Advantage of BAC: More stable than YACs, and easy to manipulate compared to YAC
Disadvantage: Less stable than BAC and produce chimeric effects (deletion and inversion of cloned sequences)	Disadvantage: Small insert size compared to YAC Reference: Bajpai B. (2013). High Capacity Vectors. Advances in Biotechnology, 1–10.

A vector is any DNA molecule that has the ability to replicate inside the host to which the desired gene has integrated for cloning.

Vectors include plasmids, bacteriophages, cosmids, BAC, YAC, yeast vectors, shuttle vectors etc.

YAC and BAC vectors are two genetically engineered artificial vectors designed to clone large DNA fragments. Now both these vectors are very much modified based on requirements. Let’s differentiate the basic YAC and BAC vector in this post.

YAC	BAC
YAC vector means Yeast Artificial Chromosome vectors.	BAC vector means Bacterial Artificial Chromosome Vectors.
Developed by Burke and Olson in 1987	Developed  Melsimon et al 1992
Can replicate inside yeast Used to clone DNA sequences in yeast cells	Can replicate inside bacteria used to clone DNA sequences in bacterial cells
Basic Components of YAC vector ARS (Autonomously Replicating Sequence)  – Ori; origin of replication for Yeast CEN (Centromeric) –yeast centromere for  movement of YAC vectors  to daughter cells 2 TEL (Telomeric) -Telomeric region for behaving as yeast chromosome on linearization AmpR– Selectable marker genes for selection of transformed cells BamHI and EcoRI restriction sites for inserting our gene of interest	Basic components of BAC vector(pBAC108L first developed BAC vector) OriS – ori for bacteria repE – initiation & assembly of replication  complex  parA and parB – movement of BAC to daughter cells Selectable marker –Chloramphenicol Resistance for selection of transformed cells T7 and SP6 – Promote transcription of insert gene
Its construction is based on regions of yeast chromosome like ARS	BAC is a DNA construct, based on functional fertility plasmid (or F-plasmid), of  E. coli.
Linear vector inside yeast cell with 2 telomeric ends  just functions as yeast chromosomes without disturbing original chromosomes	Circular vector inside bacterium
Clone DNA fragments larger than 100 kb and up to 3,000 kb	Insert size is 150–350 kb.
It is used for physical mapping and sequencing of genomes. YAC libraries of human and Caenorhabditis elegans and many genomes First used in Human genome project later shifted to BAC vectors due to stability issues with YAC	It is used for physical mapping and sequencing of genomes. Used in HGP and in physical genetic maps of including Mus musculus, Arabidopsis thaliana, and Homo sapiens.
Advantage of YAC: large insert size	Advantage of BAC: More stable than YACs, and easy to manipulate compared to YAC
Disadvantage: Less stable than BAC and produce chimeric effects (deletion and inversion of cloned sequences)	Disadvantage: Small insert size compared to YAC Reference: Bajpai B. (2013). High Capacity Vectors. Advances in Biotechnology, 1–10.

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5 Differences between Viroids and Prions

5 Differences between Viroids and Prions

Both Viroids and Prions are strict intracellular, sub cellular infectious agents, always requires a living host for replication.

Viroids are the smallest known infectious agents, discovered by T.O. Diener. The first discovered is Potato spindle Tuber Viroid (PSTV).

Prions are infectious misfolded protein particles discovered by Stanley Prusiner in 1982. 

Viroids (virus like)	Prions (infectious protein)
Infectious RNA particles without protein coat	Infectious protein particles
1971, T.O.  Diener discovered PSTV	Discovered by Stanley B Prusiner, 1982
Chemical nature: Always small circular ss RNA molecule ~5-10 times smaller than the smallest DNA and RNA viral genomes.  PSTV consist of 359 nucleotides (246 to 463 nucleotide)	PrPC (prion protein cellular) has  209 amino acids present on nerve cell surface has neuro protective function. PrPsc(prion protein scrapie) protein highy stable structure with beta sheets accumulates within nerve cells and ultimately kill neurons
Viroid replication proceeds through an RNA-based rolling-circle mechanism using RNA polymerase II of host	PrPSc molecule binds to a single PrPC molecule and catalyzes its conversion into PrPSc Heterodimer model & Fibril Model
Infects only plants	Infects only animals but presence reported in plants causing nuerodegenerative disease.
Examples: Potato spindle tuber viroids (PSTV) (1st viroid discovered) Tomato plant macho viroid (TPMVd) Avocado sunblotch viroid (ASBVd) Peach latent mosaic viroid (PLMVd)	Bovine spongiform encephalopathy (BSE)—"mad cow disease", Scrapie in sheep. Human prion diseases Creutzfeldt–Jakob disease (CJD), Kuru disease

Learn More Virus vs Viroid

Both Viroids and Prions are strict intracellular, sub cellular infectious agents, always requires a living host for replication.

Viroids are the smallest known infectious agents, discovered by T.O. Diener. The first discovered is Potato spindle Tuber Viroid (PSTV).

Prions are infectious misfolded protein particles discovered by Stanley Prusiner in 1982. 

Viroids (virus like)	Prions (infectious protein)
Infectious RNA particles without protein coat	Infectious protein particles
1971, T.O.  Diener discovered PSTV	Discovered by Stanley B Prusiner, 1982
Chemical nature: Always small circular ss RNA molecule ~5-10 times smaller than the smallest DNA and RNA viral genomes.  PSTV consist of 359 nucleotides (246 to 463 nucleotide)	PrPC (prion protein cellular) has  209 amino acids present on nerve cell surface has neuro protective function. PrPsc(prion protein scrapie) protein highy stable structure with beta sheets accumulates within nerve cells and ultimately kill neurons
Viroid replication proceeds through an RNA-based rolling-circle mechanism using RNA polymerase II of host	PrPSc molecule binds to a single PrPC molecule and catalyzes its conversion into PrPSc Heterodimer model & Fibril Model
Infects only plants	Infects only animals but presence reported in plants causing nuerodegenerative disease.
Examples: Potato spindle tuber viroids (PSTV) (1st viroid discovered) Tomato plant macho viroid (TPMVd) Avocado sunblotch viroid (ASBVd) Peach latent mosaic viroid (PLMVd)	Bovine spongiform encephalopathy (BSE)—"mad cow disease", Scrapie in sheep. Human prion diseases Creutzfeldt–Jakob disease (CJD), Kuru disease

Learn More Virus vs Viroid

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Definition of Trophic Cascade? Top down vs Bottom up Trophic Cascade

Definition of Trophic Cascade? Top down vs Bottom up Trophic Cascade

What is a Trophic Cascade? Top down vs Bottom up Trophic Cascade

Trophic cascade is an ecological phenomenon that has significant effect on the overall structure and stability of an ecosystem.The term trophic cascade was coined by the famous American ecologist Robert Paine.

Trophic cascade is an indirect effect by predators that changes biomass, abundance or productivity of a population, community at successively lower trophic levels or trophic level across more than one link in a food chain.

These are powerful indirect interactions that can control the entire ecosystem.

The most common type of trophic cascade is three level trophic cascade that involves three trophic levels or feeding levels.

Top down Trophic cascade

Here the predators influence the abundance or biomass of a population at lower trophic levels.

Take this example. In the above figure, the tiger population (predator) has direct effect on the abundance of prey population. By controlling the prey population the predator indirectly influences the abundance or productivity at the producer level. This type of indirect influence at lower trophic levels by predators is termed as Top down trophic cascade.

Bottom up Trophic cascade

Here the Space or nutrients influence the abundance or biomass of a population at higher trophic levels.

In the above figure, lack of space or nutrients may lead to poor productivity at the producer level (amount of grass, no of fruits etc). This limits the number of prey population that will influence the number of predators. This type of indirect influence on higher trophic levels by lack of space or nutrients is termed as bottom up trophic cascade.

What is a Trophic Cascade? Top down vs Bottom up Trophic Cascade

Trophic cascade is an ecological phenomenon that has significant effect on the overall structure and stability of an ecosystem.The term trophic cascade was coined by the famous American ecologist Robert Paine.

Trophic cascade is an indirect effect by predators that changes biomass, abundance or productivity of a population, community at successively lower trophic levels or trophic level across more than one link in a food chain.

These are powerful indirect interactions that can control the entire ecosystem.

The most common type of trophic cascade is three level trophic cascade that involves three trophic levels or feeding levels.

Top down Trophic cascade

Here the predators influence the abundance or biomass of a population at lower trophic levels.

Take this example. In the above figure, the tiger population (predator) has direct effect on the abundance of prey population. By controlling the prey population the predator indirectly influences the abundance or productivity at the producer level. This type of indirect influence at lower trophic levels by predators is termed as Top down trophic cascade.

Bottom up Trophic cascade

Here the Space or nutrients influence the abundance or biomass of a population at higher trophic levels.

In the above figure, lack of space or nutrients may lead to poor productivity at the producer level (amount of grass, no of fruits etc). This limits the number of prey population that will influence the number of predators. This type of indirect influence on higher trophic levels by lack of space or nutrients is termed as bottom up trophic cascade.

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10 Differences between Positive sense RNA Viruses and Negative sense ss RNA Viruses

10 Differences between Positive sense RNA Viruses and Negative sense ss RNA Viruses

Based on the sense or polarity of the genome, single stranded RNA viruses are of two types.

1. Positive Sense RNA viruses (+ssRNA virus) or PSV

2. Negative Sense ss RNA viruses (-ssRNA virus) or NSV

Positive sense RNA viruses (PSV)	Negative sense ss RNA viruses (NSV)
Genetic Material is positive sense ssRNA	Genetic Material is Negative sense ssRNA
Virus with +ssRNA genome can be translated directly to make viral proteins  by host ribosome	Virus with -ssRNA genome cannot be translated directly to make viral proteins.
‘translation ready genome’	Not ‘translational ready genome’
Viral genome acts like cellular mRNA. ssRNA has 5’cap and poly A tail for recognition by eukaryotic host ribosme.	Negative ssRNA is  complementary to mRNA
Genetic material is infectious inside the host	Genetic material is not infectious inside the host. It It should be converted to +ss RNA by viral RNA dependent RNA polymerase (RdRP) for viral protein synthesis inside host.
+ve ssRNA viruses belong to Group IV in the Baltimore classification.	-ve  sense ssRNA viruses belong to Group V in the Baltimore classification.
The genome  usually contains relatively few genes, including an RdRP.	Negative-sense single-stranded RNA viruses have complex genomic sequences, cell cycles, and mode of replication
Most common type of plant viruses and more abundant	Less abundant compared to +ve ssRNA viruses and more infectious
Examples: SARS CoV-2, Rhino viruses, Dengue virus, MERS corona virus.	Examples: Influenza virus, Ebola virus, Hanta virus, Rabies virus, Mumps virus

Based on the sense or polarity of the genome, single stranded RNA viruses are of two types.

1. Positive Sense RNA viruses (+ssRNA virus) or PSV

2. Negative Sense ss RNA viruses (-ssRNA virus) or NSV

Positive sense RNA viruses (PSV)	Negative sense ss RNA viruses (NSV)
Genetic Material is positive sense ssRNA	Genetic Material is Negative sense ssRNA
Virus with +ssRNA genome can be translated directly to make viral proteins  by host ribosome	Virus with -ssRNA genome cannot be translated directly to make viral proteins.
‘translation ready genome’	Not ‘translational ready genome’
Viral genome acts like cellular mRNA. ssRNA has 5’cap and poly A tail for recognition by eukaryotic host ribosme.	Negative ssRNA is  complementary to mRNA
Genetic material is infectious inside the host	Genetic material is not infectious inside the host. It It should be converted to +ss RNA by viral RNA dependent RNA polymerase (RdRP) for viral protein synthesis inside host.
+ve ssRNA viruses belong to Group IV in the Baltimore classification.	-ve  sense ssRNA viruses belong to Group V in the Baltimore classification.
The genome  usually contains relatively few genes, including an RdRP.	Negative-sense single-stranded RNA viruses have complex genomic sequences, cell cycles, and mode of replication
Most common type of plant viruses and more abundant	Less abundant compared to +ve ssRNA viruses and more infectious
Examples: SARS CoV-2, Rhino viruses, Dengue virus, MERS corona virus.	Examples: Influenza virus, Ebola virus, Hanta virus, Rabies virus, Mumps virus

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Difference between Coronavirus and COVID-19

Difference between Coronavirus and COVID-19

Corona Virus vs COVID-19

Corona Virus

Corona virus is a family of virus that causes diseases in mammals and birds.

According to World Health Organization (WHO), in humans, several coronaviruses are known to cause respiratory infections ranging from the common cold to more severe diseases such as Middle East Respiratory Syndrome (MERS) and Severe Acute Respiratory Syndrome (SARS).

Some examples include

a) Coronaviruses causing mild symptoms of common cold

• Human coronavirus OC43 (HCoV-OC43), Human coronavirus HKU1, Human coronavirus NL63 (HCoV-NL63, New Haven coronavirus), Human coronavirus 229E (HCoV-229E)

b) Coronaviruses causing severe respiratory infections including Pneumonia

• Middle East respiratory syndrome-related coronavirus (MERS-CoV)
• Severe acute respiratory syndrome coronavirus (SARS-CoV)
• COVID-19

Structure: Enveloped positive sense RNA viruses with nucleocapsid of helical symmetry

Why the name “corona”?

The most prominent feature of coronaviruses is the club-shape spike projections emanating from the surface of the virion. These spikes are a defining feature of the virion and give them the appearance of a solar corona under two-dimensional transmission electron microscopy (TEM), prompting the name, coronaviruses. (Latin corona meaning “crown or halo”)

COVID-19

• COVID 19 Is the infectious disease caused by newly discovered corona virus, first identified at Wuhan, China in December 2019
• February 11, 2020 WHO announced an official name  COVID-19, ‘CO’ stands for ‘CORONA,’ ‘VI’ for ‘VIrus,’ and ‘D’ for Disease
• Formerly, this disease was called as “2019 novel coronavirus” or “2019-nCoV”

Symptoms of Covid-19 include

• fever, tiredness, and dry cough. Some patients may have aches and pains, nasal congestion, runny nose, sore throat or diarrhea. These symptoms are usually mild and begin gradually.

Transmission of disease: 

• People can catch COVID-19 from others who have the virus, through respiratory droplets or through infected surfaces

Preventive measures:

• Regularly and thoroughly clean your hands with an alcohol-based hand rub or wash them with soap and water.
• Maintain at least 1 meter (3 feet) distance between yourself and anyone who is coughing or sneezing.
• Avoid touching eyes, nose and mouth as infected surfaces can spread the disease
• Cover your mouth and nose with your bent elbow or tissue when you cough or sneeze. Then dispose of the used tissue immediately.
• Avoid direct contact with animals and surfaces in contact with animals
• Stay home if you feel unwell. If you have a fever, cough and difficulty in breathing, seek medical attention immediately.

For more updates and answers on COVID-19 visit: 

https://www.who.int/news-room/q-a-detail/q-a-coronaviruses

Reference:

Zhu, N., Zhang, D., Wang, W., Li, X., Yang, B., Song, J., ... & Niu, P. (2020). A novel coronavirus from patients with pneumonia in China, 2019. New England Journal of Medicine.

Fehr, A. R., & Perlman, S. (2015). Coronaviruses: an overview of their replication and pathogenesis. In Coronaviruses (pp. 1-23). Humana Press, New York, NY.

Corona Virus vs COVID-19

Corona Virus

Corona virus is a family of virus that causes diseases in mammals and birds.

According to World Health Organization (WHO), in humans, several coronaviruses are known to cause respiratory infections ranging from the common cold to more severe diseases such as Middle East Respiratory Syndrome (MERS) and Severe Acute Respiratory Syndrome (SARS).

Some examples include

a) Coronaviruses causing mild symptoms of common cold

• Human coronavirus OC43 (HCoV-OC43), Human coronavirus HKU1, Human coronavirus NL63 (HCoV-NL63, New Haven coronavirus), Human coronavirus 229E (HCoV-229E)

b) Coronaviruses causing severe respiratory infections including Pneumonia

• Middle East respiratory syndrome-related coronavirus (MERS-CoV)
• Severe acute respiratory syndrome coronavirus (SARS-CoV)
• COVID-19

Structure: Enveloped positive sense RNA viruses with nucleocapsid of helical symmetry

Why the name “corona”?

The most prominent feature of coronaviruses is the club-shape spike projections emanating from the surface of the virion. These spikes are a defining feature of the virion and give them the appearance of a solar corona under two-dimensional transmission electron microscopy (TEM), prompting the name, coronaviruses. (Latin corona meaning “crown or halo”)

COVID-19

• COVID 19 Is the infectious disease caused by newly discovered corona virus, first identified at Wuhan, China in December 2019
• February 11, 2020 WHO announced an official name  COVID-19, ‘CO’ stands for ‘CORONA,’ ‘VI’ for ‘VIrus,’ and ‘D’ for Disease
• Formerly, this disease was called as “2019 novel coronavirus” or “2019-nCoV”

Symptoms of Covid-19 include

• fever, tiredness, and dry cough. Some patients may have aches and pains, nasal congestion, runny nose, sore throat or diarrhea. These symptoms are usually mild and begin gradually.

Transmission of disease: 

• People can catch COVID-19 from others who have the virus, through respiratory droplets or through infected surfaces

Preventive measures:

• Regularly and thoroughly clean your hands with an alcohol-based hand rub or wash them with soap and water.
• Maintain at least 1 meter (3 feet) distance between yourself and anyone who is coughing or sneezing.
• Avoid touching eyes, nose and mouth as infected surfaces can spread the disease
• Cover your mouth and nose with your bent elbow or tissue when you cough or sneeze. Then dispose of the used tissue immediately.
• Avoid direct contact with animals and surfaces in contact with animals
• Stay home if you feel unwell. If you have a fever, cough and difficulty in breathing, seek medical attention immediately.

For more updates and answers on COVID-19 visit: 

https://www.who.int/news-room/q-a-detail/q-a-coronaviruses

Reference:

Zhu, N., Zhang, D., Wang, W., Li, X., Yang, B., Song, J., ... & Niu, P. (2020). A novel coronavirus from patients with pneumonia in China, 2019. New England Journal of Medicine.

Fehr, A. R., & Perlman, S. (2015). Coronaviruses: an overview of their replication and pathogenesis. In Coronaviruses (pp. 1-23). Humana Press, New York, NY.

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Difference between Cell Theory and Modern Cell Theory

Difference between Cell Theory and Modern Cell Theory

Cell Theory 1839

Proposed by Theodor Schwann (1810–1882) and Matthias Jakob Schleiden (1804–1881)

1. All living organisms are composed of one or more cells.

2. The cell is the basic structural and functional unit of all living things.

The third tenet was proposed by Rudolf Virchow, a German Pathologist (1821–1902) in 1855

3. Cells arise from pre-existing cells “Omnis cellula e cellula” (by cell division; mitosis or meiosis; not derived from spontaneous generation)

Background:

Schleiden in 1838, German botanist, found out cell as the basic unit of plant structure.

Theodor Schwann, a German physiologist in 1839 defined cell as the basic unit of animal structure also. In 1839, they together proposed the two statements of the present cell theory.

5 Examples of Exceptions to Cell Theory

Modern Cell Theory

The knowledge of the advancement in the field of molecular biology, biochemistry, etc. is considered to make the following additions to the above classical cell theory

4. Cells contain heredity information in their DNA. This information is passed to new cells by cell division.

5. All cells have the same basic chemical composition.

6. Energy flow (Metabolism and biochemistry) occurs within cells.

The other major contributors to the Cell Theory are Robert Hooke; who coined the tern “cell” and Antonie van Leeuwenhoek; who was the first one to observe living organisms under his own microscope.

Cell Theory 1839

Proposed by Theodor Schwann (1810–1882) and Matthias Jakob Schleiden (1804–1881)

1. All living organisms are composed of one or more cells.

2. The cell is the basic structural and functional unit of all living things.

The third tenet was proposed by Rudolf Virchow, a German Pathologist (1821–1902) in 1855

3. Cells arise from pre-existing cells “Omnis cellula e cellula” (by cell division; mitosis or meiosis; not derived from spontaneous generation)

Background:

Schleiden in 1838, German botanist, found out cell as the basic unit of plant structure.

Theodor Schwann, a German physiologist in 1839 defined cell as the basic unit of animal structure also. In 1839, they together proposed the two statements of the present cell theory.

5 Examples of Exceptions to Cell Theory

Modern Cell Theory

The knowledge of the advancement in the field of molecular biology, biochemistry, etc. is considered to make the following additions to the above classical cell theory

4. Cells contain heredity information in their DNA. This information is passed to new cells by cell division.

5. All cells have the same basic chemical composition.

6. Energy flow (Metabolism and biochemistry) occurs within cells.

The other major contributors to the Cell Theory are Robert Hooke; who coined the tern “cell” and Antonie van Leeuwenhoek; who was the first one to observe living organisms under his own microscope.

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10 Differences between chemiosmosis in Cellular Respiration and Photosynthesis

10 Differences between chemiosmosis in Cellular Respiration and Photosynthesis

Chemiosmosis in Cellular Respiration vs chemiosmosis in Photosynthesis

Chemiosmotic Hypothesis was proposed by Peter Mitchell 1961. He was awarded the Nobel Prize for Chemistry in 1978. This process is occurring during cellular respiration and photosynthesis.

Chemiosmotic Theory states that Electron transport and ATP synthesis are coupled by a proton gradient across the inner mitochondrial membrane.

Peter Mitchell proposed this theory to explain ATP synthesis during cellular respiration

See simple step wise explanation on

Chemiosmosis in Photosynthesis

Chemiosmosis in Cellular Respiration

Chemiosmosis in Cellular Respiration	Chemiosmosis in Photosynthesis
Chemiosmosis in Cellular Respiration occurs in all living cells.	Chemiosmosis in photosynthesis occurs only in green plants and cyanobacteria
Chemiosmosis occurs during Electron transport chain of cellular respiration	Chemiosmosis occurs during Light dependent reaction of photosynthesis
The organelle involved is Mitochondrion	The organelle involved is Chloroplast
The exact site of chemiosmosis is Mitochondrial inner membrane (cristae)	The exact site of chemiosmosis is thylakoid membrane of chloroplast
Electron flows through electron carriers located in the Mitochondrial inner membrane	Electron flows through electron carriers located in the Thylakoid membrane of chloroplast
Proton gradient formation occurs across the mitochondrial inner membrane	Proton gradient formation occurs across thylakoid membrane
Proton (H+) is pumped from matrix into the intermembrane space of mitochondria using energy derived from electron flow	Proton (H+) is pumped from stroma into the thylakoid lumen or thylakoid space using energy derived from electron flow
ATP synthesis occurs towards the matrix side as ATP synthase is oriented towards matrix side	ATP synthesis occurs towards the stromal side as ATP synthase is oriented towards stromal side
Transforms chemical energy or bond energy form food to ATP	Transforms light energy into chemical energy in the form of ATP
ATP synthesized is used to drive all cellular activities	ATP synthesized is used to fix carbondioxide to carbohydrates during light independent reaction of photosynthesis

Chemiosmosis in Cellular Respiration vs chemiosmosis in Photosynthesis

Chemiosmotic Hypothesis was proposed by Peter Mitchell 1961. He was awarded the Nobel Prize for Chemistry in 1978. This process is occurring during cellular respiration and photosynthesis.

Chemiosmotic Theory states that Electron transport and ATP synthesis are coupled by a proton gradient across the inner mitochondrial membrane.

Peter Mitchell proposed this theory to explain ATP synthesis during cellular respiration

See simple step wise explanation on

Chemiosmosis in Photosynthesis

Chemiosmosis in Cellular Respiration

Chemiosmosis in Cellular Respiration	Chemiosmosis in Photosynthesis
Chemiosmosis in Cellular Respiration occurs in all living cells.	Chemiosmosis in photosynthesis occurs only in green plants and cyanobacteria
Chemiosmosis occurs during Electron transport chain of cellular respiration	Chemiosmosis occurs during Light dependent reaction of photosynthesis
The organelle involved is Mitochondrion	The organelle involved is Chloroplast
The exact site of chemiosmosis is Mitochondrial inner membrane (cristae)	The exact site of chemiosmosis is thylakoid membrane of chloroplast
Electron flows through electron carriers located in the Mitochondrial inner membrane	Electron flows through electron carriers located in the Thylakoid membrane of chloroplast
Proton gradient formation occurs across the mitochondrial inner membrane	Proton gradient formation occurs across thylakoid membrane
Proton (H+) is pumped from matrix into the intermembrane space of mitochondria using energy derived from electron flow	Proton (H+) is pumped from stroma into the thylakoid lumen or thylakoid space using energy derived from electron flow
ATP synthesis occurs towards the matrix side as ATP synthase is oriented towards matrix side	ATP synthesis occurs towards the stromal side as ATP synthase is oriented towards stromal side
Transforms chemical energy or bond energy form food to ATP	Transforms light energy into chemical energy in the form of ATP
ATP synthesized is used to drive all cellular activities	ATP synthesized is used to fix carbondioxide to carbohydrates during light independent reaction of photosynthesis

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5 Differences between Proteinogenic Amino Acid and Non-proteinogenic amino acids

5 Differences between Proteinogenic Amino Acid and Non-proteinogenic amino acids

Proteinogenic vs Non-proteinogenic amino acids

Proteins are nitrogen containing bio molecules made up of amino acids joined by peptide bond.

An amino acids consists of a central α carbon atom joined by 4 groups namely Hydrogen (H), amino group (–NH2), carboxyl group (-COOH) and side group (R-group).

Proteinogenic amino acids are “protein forming amino acids where as non-proteinogenic amino acids not naturally incorporated into proteins

Proteinogenic amino acids	Non-proteinogenic aminoacids
Proteinogenic amino acids are those which are naturally encoded in the genetic code of any organism	Non-coded or non-proteinogenic amino acids are those not naturally encoded or found in the genetic code of any organism.
Natural amino acids incorporated into proteins during translation	Amino acids not incorporated into proteins during translation
Protein forming amino acids or amino acids that are natural constituents of proteins	Not Protein forming amino acids or amino acids that are not naturally incorporated into proteins
Coded amino acids or proteinogenic amino acids are those which are naturally encoded in the genetic code of any organism	Non-coded or non-proteinogenic amino acids are those not naturally encoded or found in the genetic code of any organism.
Examples: In Eukaryotes all 21 amino acids including selenocysteine are  proteinogenic. (Glycine, alanine, valine etc)	Ornithine, citruline, Gamma-Aminobutyric Acid (GABA) etc
Forms all proteins that carry out different activities of the cell like enzymes, hormones like insulin. See 8 different functions of proteins here	They are often intermediates in biosynthesis with specific physiological functions. For example GABA is a neurotransmitter that blocks impulses between nerve cells in the brain.

Proteinogenic vs Non-proteinogenic amino acids

Proteins are nitrogen containing bio molecules made up of amino acids joined by peptide bond.

An amino acids consists of a central α carbon atom joined by 4 groups namely Hydrogen (H), amino group (–NH2), carboxyl group (-COOH) and side group (R-group).

Proteinogenic amino acids are “protein forming amino acids where as non-proteinogenic amino acids not naturally incorporated into proteins

Proteinogenic amino acids	Non-proteinogenic aminoacids
Proteinogenic amino acids are those which are naturally encoded in the genetic code of any organism	Non-coded or non-proteinogenic amino acids are those not naturally encoded or found in the genetic code of any organism.
Natural amino acids incorporated into proteins during translation	Amino acids not incorporated into proteins during translation
Protein forming amino acids or amino acids that are natural constituents of proteins	Not Protein forming amino acids or amino acids that are not naturally incorporated into proteins
Coded amino acids or proteinogenic amino acids are those which are naturally encoded in the genetic code of any organism	Non-coded or non-proteinogenic amino acids are those not naturally encoded or found in the genetic code of any organism.
Examples: In Eukaryotes all 21 amino acids including selenocysteine are  proteinogenic. (Glycine, alanine, valine etc)	Ornithine, citruline, Gamma-Aminobutyric Acid (GABA) etc
Forms all proteins that carry out different activities of the cell like enzymes, hormones like insulin. See 8 different functions of proteins here	They are often intermediates in biosynthesis with specific physiological functions. For example GABA is a neurotransmitter that blocks impulses between nerve cells in the brain.

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