5 Differences between Proteinogenic Amino Acid and Non-proteinogenic amino acids

5 Differences between Proteinogenic Amino Acid and Non-proteinogenic amino acids

Proteinogenic vs Non-proteinogenic amino acids

Proteins are nitrogen containing bio molecules made up of amino acids joined by peptide bond.

An amino acids consists of a central α carbon atom joined by 4 groups namely Hydrogen (H), amino group (–NH2), carboxyl group (-COOH) and side group (R-group).

Proteinogenic amino acids are “protein forming amino acids where as non-proteinogenic amino acids not naturally incorporated into proteins

Proteinogenic amino acids	Non-proteinogenic aminoacids
Proteinogenic amino acids are those which are naturally encoded in the genetic code of any organism	Non-coded or non-proteinogenic amino acids are those not naturally encoded or found in the genetic code of any organism.
Natural amino acids incorporated into proteins during translation	Amino acids not incorporated into proteins during translation
Protein forming amino acids or amino acids that are natural constituents of proteins	Not Protein forming amino acids or amino acids that are not naturally incorporated into proteins
Coded amino acids or proteinogenic amino acids are those which are naturally encoded in the genetic code of any organism	Non-coded or non-proteinogenic amino acids are those not naturally encoded or found in the genetic code of any organism.
Examples: In Eukaryotes all 21 amino acids including selenocysteine are  proteinogenic. (Glycine, alanine, valine etc)	Ornithine, citruline, Gamma-Aminobutyric Acid (GABA) etc
Forms all proteins that carry out different activities of the cell like enzymes, hormones like insulin. See 8 different functions of proteins here	They are often intermediates in biosynthesis with specific physiological functions. For example GABA is a neurotransmitter that blocks impulses between nerve cells in the brain.

Proteinogenic vs Non-proteinogenic amino acids

Proteins are nitrogen containing bio molecules made up of amino acids joined by peptide bond.

An amino acids consists of a central α carbon atom joined by 4 groups namely Hydrogen (H), amino group (–NH2), carboxyl group (-COOH) and side group (R-group).

Proteinogenic amino acids are “protein forming amino acids where as non-proteinogenic amino acids not naturally incorporated into proteins

Proteinogenic amino acids	Non-proteinogenic aminoacids
Proteinogenic amino acids are those which are naturally encoded in the genetic code of any organism	Non-coded or non-proteinogenic amino acids are those not naturally encoded or found in the genetic code of any organism.
Natural amino acids incorporated into proteins during translation	Amino acids not incorporated into proteins during translation
Protein forming amino acids or amino acids that are natural constituents of proteins	Not Protein forming amino acids or amino acids that are not naturally incorporated into proteins
Coded amino acids or proteinogenic amino acids are those which are naturally encoded in the genetic code of any organism	Non-coded or non-proteinogenic amino acids are those not naturally encoded or found in the genetic code of any organism.
Examples: In Eukaryotes all 21 amino acids including selenocysteine are  proteinogenic. (Glycine, alanine, valine etc)	Ornithine, citruline, Gamma-Aminobutyric Acid (GABA) etc
Forms all proteins that carry out different activities of the cell like enzymes, hormones like insulin. See 8 different functions of proteins here	They are often intermediates in biosynthesis with specific physiological functions. For example GABA is a neurotransmitter that blocks impulses between nerve cells in the brain.

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10 Difference between Hexokinase and Glucokinase

10 Difference between Hexokinase and Glucokinase

Both Hexokinase and Glucokinase are enzymes catalyzing the phophorylation of Glucose to Glucose-6-phosphate using ATP. During the reaction, one ATP molecule is cleaved to ADP and the phosphate thus released is added to glucose. Hexokinase and Glucokinae are isoenzymes with same catalytic activity but have different physical properties and site of action. Glucokinase is also called as human hexokinase IV, hexokinase D etc

Hexokinase	Glucokinase (Hexokinase D)
Present in all tissues except the liver and the Beta cells of pancreas	Present in liver and Beta cells of pancreas
Acts upon many hexoses such as fructose, galactose including glucose	The only substrate is D-glucose
Hexokinase is one of the regulatory enzymes of glycolysis	Glucokinase plays a central role as a glucose sensor in the regulation of glucose homeostasis.
Hexokinase has high Km value that is high affinity for the substrate glucose	Glucokinase has high Km value that means low affinity for the substrate
The maximum reaction rate (Vmax) of hexokinase is low that means it gets saturated quickly by increasing glucose concentration	The maximum reaction rate (Vmax) of glucokinase is quite high, thus can handle larger glucose load resulting in a rapid conversion of glucose into usable energy.
Hexokinase is active even at low glucose levels	Glucokinase is active only at high glucose levels in liver
Is not inducible (*constitutive enzyme)	Is induced by glucose and insulin
Hexokinase is an allosteric enzyme with **allosteric site for regulation of enzyme activity	Glucokinase is not an allosteric enzyme
Feedback inhibition of hexokinase by glucose 6 phosphate (product)	No direct feedback inhibition; not inhibited by glucose-6-phosphate

*Constitutive enzymes are always produced in constant amounts without regard to the physiological demand or the concentration of the substrate.

**Allosteric site: is the site other than the active site where effector molecule binds and regulated enzyme activity. This type of regulation is called allosteric regulation.

Both Hexokinase and Glucokinase are enzymes catalyzing the phophorylation of Glucose to Glucose-6-phosphate using ATP. During the reaction, one ATP molecule is cleaved to ADP and the phosphate thus released is added to glucose. Hexokinase and Glucokinae are isoenzymes with same catalytic activity but have different physical properties and site of action. Glucokinase is also called as human hexokinase IV, hexokinase D etc

Hexokinase	Glucokinase (Hexokinase D)
Present in all tissues except the liver and the Beta cells of pancreas	Present in liver and Beta cells of pancreas
Acts upon many hexoses such as fructose, galactose including glucose	The only substrate is D-glucose
Hexokinase is one of the regulatory enzymes of glycolysis	Glucokinase plays a central role as a glucose sensor in the regulation of glucose homeostasis.
Hexokinase has high Km value that is high affinity for the substrate glucose	Glucokinase has high Km value that means low affinity for the substrate
The maximum reaction rate (Vmax) of hexokinase is low that means it gets saturated quickly by increasing glucose concentration	The maximum reaction rate (Vmax) of glucokinase is quite high, thus can handle larger glucose load resulting in a rapid conversion of glucose into usable energy.
Hexokinase is active even at low glucose levels	Glucokinase is active only at high glucose levels in liver
Is not inducible (*constitutive enzyme)	Is induced by glucose and insulin
Hexokinase is an allosteric enzyme with **allosteric site for regulation of enzyme activity	Glucokinase is not an allosteric enzyme
Feedback inhibition of hexokinase by glucose 6 phosphate (product)	No direct feedback inhibition; not inhibited by glucose-6-phosphate

*Constitutive enzymes are always produced in constant amounts without regard to the physiological demand or the concentration of the substrate.

**Allosteric site: is the site other than the active site where effector molecule binds and regulated enzyme activity. This type of regulation is called allosteric regulation.

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Difference between El Nino and La Nina (El Nino vs La-Nina)

Difference between El Nino and La Nina (El Nino vs La-Nina)

El Nino

     1. El Nino is warming of the Pacific Ocean between South America and the Data Line.

     2. It accompanies high air surface pressure in the western Pacific.

3. El Nino occurs when tropical Pacific ocean trade winds die out and ocean temperatures become usually worm.

La-Nina

1. La Nina exists when cooler than usual ocean temperatures occur on the equator between South America and the Data Line.
2.   It accompanies low air surface pressure in the Eastern Pacific.
3.   La Nina, which occurs when the trade winds blow unusually hard and the sea temperature becomes colder than normal.

El Nino

     1. El Nino is warming of the Pacific Ocean between South America and the Data Line.

     2. It accompanies high air surface pressure in the western Pacific.

3. El Nino occurs when tropical Pacific ocean trade winds die out and ocean temperatures become usually worm.

La-Nina

1. La Nina exists when cooler than usual ocean temperatures occur on the equator between South America and the Data Line.
2.   It accompanies low air surface pressure in the Eastern Pacific.
3.   La Nina, which occurs when the trade winds blow unusually hard and the sea temperature becomes colder than normal.

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10 Differences between Calvin Cycle and Krebs Cycle (C3 Cycle vs Citric Acid Cycle)

10 Differences between Calvin Cycle and Krebs Cycle (C3 Cycle vs Citric Acid Cycle)

Difference between C3 cycle and citric acid cycle (Calvin cycle and Krebs cycle)

We have already discussed the difference between C3 and C4 cycle, C3, C4 and CAM cycle. In this post we are discussing the difference between Calvin Cycle or C3 cycle in Photosynthesis and Krebs cycle in Cellular Respiration

Calvin Cycle or C3 cycle	Krebs Cycle or Citric Acid Cycle
A stage in photosynthesis where CO2 is fixed to carbohydrate using energy (ATP and NADPH) produced during light reaction	A stage in cellular respiration that involves series of reactions that produces carbon dioxide molecules, GTP/ATP and reduced forms of NADH and FADH2.
An anabolic process where carbohydrate is synthesized	An catabolic process where respiratory substrates such as carbohydrates, fats etc are broken down releasing energy
Site of reaction is stroma of chloroplast	Site of reaction is matrix of mitochondrion
Occur in plants	Takes place in all aerobic organisms including plants
Anaerobic process (oxygen not involved)	Aerobic process that involves oxygen in Electron transport chain which is essential for running Krebs cycle
Produces glucose using energy	Oxidizes glucose releasing energy
The first stable compound is 3 carbon phosphoglyceric acid	The first stable compound is 6 carbon Citric acid
RuBisCO is the first enzyme of the Calvin cycle	Citrate synthase is the first enzyme of the citric acid cycle
ATP and CO2 used in the cycle	ATP and CO2 produced in the cycle
Carbohydrate is synthesized	1 GTP/ATP, 3 NADH + H+, 1FADH2 & 2 carbon dioxide molecule per turn of cycle

Difference between C3 cycle and citric acid cycle (Calvin cycle and Krebs cycle)

We have already discussed the difference between C3 and C4 cycle, C3, C4 and CAM cycle. In this post we are discussing the difference between Calvin Cycle or C3 cycle in Photosynthesis and Krebs cycle in Cellular Respiration

Calvin Cycle or C3 cycle	Krebs Cycle or Citric Acid Cycle
A stage in photosynthesis where CO2 is fixed to carbohydrate using energy (ATP and NADPH) produced during light reaction	A stage in cellular respiration that involves series of reactions that produces carbon dioxide molecules, GTP/ATP and reduced forms of NADH and FADH2.
An anabolic process where carbohydrate is synthesized	An catabolic process where respiratory substrates such as carbohydrates, fats etc are broken down releasing energy
Site of reaction is stroma of chloroplast	Site of reaction is matrix of mitochondrion
Occur in plants	Takes place in all aerobic organisms including plants
Anaerobic process (oxygen not involved)	Aerobic process that involves oxygen in Electron transport chain which is essential for running Krebs cycle
Produces glucose using energy	Oxidizes glucose releasing energy
The first stable compound is 3 carbon phosphoglyceric acid	The first stable compound is 6 carbon Citric acid
RuBisCO is the first enzyme of the Calvin cycle	Citrate synthase is the first enzyme of the citric acid cycle
ATP and CO2 used in the cycle	ATP and CO2 produced in the cycle
Carbohydrate is synthesized	1 GTP/ATP, 3 NADH + H+, 1FADH2 & 2 carbon dioxide molecule per turn of cycle

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5 Differences between Genetics and Genomics (Genetics vs Genomics)

5 Differences between Genetics and Genomics (Genetics vs Genomics)

Genetics is a branch of biology concerned with the study of genes, genetic variation, and heredity in organisms.

Definition: Genomics is an multidisciplinary branch of biology focusing on the study the entire set of genes (genome) in an organism. You can watch the video also on Genetics vs genomics for better understanding

Genetics	Genomics
Genetics is the study of single genes and their heredity and variation in organisms	Genomics is the study of all genes (the genome) of a person or  an organisms, including interactions of those genes with each other and with the person's/organisms environment.
Genetics is often a study of single gene or a few gene and their effects	Genomics is the study of complex diseases caused by multiple genes and environmental factors
Genetics is a classical branch of biology dates back to Mendel’s work on pea plants during early 1800, but rediscovered only during 1900.	Genomics is a much newer field flourished within last two decades due to technical advances in DNA sequencing and computational biology.
In 1906, William Bateson coined the term  “genetics” for the discipline of biology specifically dedicated to study heredity and variation.	The term genomics was coined in 1986 by Tom Roderick, a geneticist at the Jackson Laboratory in Maine, during a meeting about the mapping of the human genome.
Examples of genetic or inherited disorders include cystic fibrosis, Huntington's disease etc caused by mutation in a single gene	Genomics includes the scientific study of complex diseases such as heart disease, obesity, asthma, diabetes and cancer. (Multi gene diseases)
Primarily focussing on the study and treatment of single gene inherited disorders	Primarily focussing on the study and treatment of complex diseases caused by multiple genes and environmental factors
Genetics uses crossing, genomic mutation, recombination etc to study a trait associated with a gene	Genomics uses a combination of recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble and analyze the structure and function of genomes.

Reference:

https://www.genome.gov/about-genomics/fact-sheets/Genetics-vs-Genomics

https://www.ebi.ac.uk/training/online/course/genomics-introduction-ebi-resources/what-genomics

Genetics is a branch of biology concerned with the study of genes, genetic variation, and heredity in organisms.

Definition: Genomics is an multidisciplinary branch of biology focusing on the study the entire set of genes (genome) in an organism. You can watch the video also on Genetics vs genomics for better understanding

Genetics	Genomics
Genetics is the study of single genes and their heredity and variation in organisms	Genomics is the study of all genes (the genome) of a person or  an organisms, including interactions of those genes with each other and with the person's/organisms environment.
Genetics is often a study of single gene or a few gene and their effects	Genomics is the study of complex diseases caused by multiple genes and environmental factors
Genetics is a classical branch of biology dates back to Mendel’s work on pea plants during early 1800, but rediscovered only during 1900.	Genomics is a much newer field flourished within last two decades due to technical advances in DNA sequencing and computational biology.
In 1906, William Bateson coined the term  “genetics” for the discipline of biology specifically dedicated to study heredity and variation.	The term genomics was coined in 1986 by Tom Roderick, a geneticist at the Jackson Laboratory in Maine, during a meeting about the mapping of the human genome.
Examples of genetic or inherited disorders include cystic fibrosis, Huntington's disease etc caused by mutation in a single gene	Genomics includes the scientific study of complex diseases such as heart disease, obesity, asthma, diabetes and cancer. (Multi gene diseases)
Primarily focussing on the study and treatment of single gene inherited disorders	Primarily focussing on the study and treatment of complex diseases caused by multiple genes and environmental factors
Genetics uses crossing, genomic mutation, recombination etc to study a trait associated with a gene	Genomics uses a combination of recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble and analyze the structure and function of genomes.

Reference:

https://www.genome.gov/about-genomics/fact-sheets/Genetics-vs-Genomics

https://www.ebi.ac.uk/training/online/course/genomics-introduction-ebi-resources/what-genomics

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Difference between Oomycetes and True fungi (Oomycetes vs True Fungi)

Difference between Oomycetes and True fungi (Oomycetes vs True Fungi)

Fungi are a group of microscopic as well as macroscopic, spore bearing, chlorophyll lacking, filamentous and heterotrophic thallophytes which reproduce asexually and sexually. Fungi are classified into slime moulds, oomycetes and true fungi. The Oomycetes (water moulds) are primarily aquatic fungi live as saprophytes or parasites. Although oomycetes morphologically similar to true fungi and exhibit absorptive nutrition and thus long classified with them, following features show that there are profound biological differences between oomycetes and true fungi.

Oomycetes	True fungi
Diploid somatic thallus	Haploid somatic thallus
Cellulose cell wall	Chitin present, No cellulose cell wall
Mitochondria cristae, tubular	Mitochondria cristate, plate like
Hydroxyprotine wall protein present	Hydroxyprotine wall protein absent
Lysine biosynthesis  Like plants, use diamino pimelic acid pathway	Like animals, use aminoadipic acid pathway

Oomycetes vs True fungi 

1. Somatic phases of oomycetes are diploid, whereas it is haploid in true fungi.

2. Meiosis occurs in developing sex organs or gametangia

3. Many produce egg cells during sexual reproduction. They are also known as egg fungi.

4. Most members produce swimming biflagellate zoospores with an anterior tinsel flagellum and a posterior whiplash flagellum.

5.The cell wall is mainly cellulosic in composition rather than chitionous as is the case in true fungi.

7. The vegetative thallus resembles the algal thallus in general construction.

8.The hyphal walls off oomycetes contain aminoacid hydroxyprotine which is not found other fungi, but is characteristic of the cell walls of green algae.

9. A number of biochemical synthetic pathways in the oomycetes are very different than those present on all other true fungi. Oomycetes like the plants synthesise lysine using diaminopimelic acid pathway rather than through the aminoadipic acid pathway.

10. Oomycetes have tubular mitochondrial cristae. All other fungi have plate like mitochondrial cristae.

11.Sexual reproduction in oomycetes in oogaamous. It takes place by gametagial contact resulting in a characteristic thick walled resting spore, called an oospore.

Fungi are a group of microscopic as well as macroscopic, spore bearing, chlorophyll lacking, filamentous and heterotrophic thallophytes which reproduce asexually and sexually. Fungi are classified into slime moulds, oomycetes and true fungi. The Oomycetes (water moulds) are primarily aquatic fungi live as saprophytes or parasites. Although oomycetes morphologically similar to true fungi and exhibit absorptive nutrition and thus long classified with them, following features show that there are profound biological differences between oomycetes and true fungi.

Oomycetes	True fungi
Diploid somatic thallus	Haploid somatic thallus
Cellulose cell wall	Chitin present, No cellulose cell wall
Mitochondria cristae, tubular	Mitochondria cristate, plate like
Hydroxyprotine wall protein present	Hydroxyprotine wall protein absent
Lysine biosynthesis  Like plants, use diamino pimelic acid pathway	Like animals, use aminoadipic acid pathway

Oomycetes vs True fungi 

1. Somatic phases of oomycetes are diploid, whereas it is haploid in true fungi.

2. Meiosis occurs in developing sex organs or gametangia

3. Many produce egg cells during sexual reproduction. They are also known as egg fungi.

4. Most members produce swimming biflagellate zoospores with an anterior tinsel flagellum and a posterior whiplash flagellum.

5.The cell wall is mainly cellulosic in composition rather than chitionous as is the case in true fungi.

7. The vegetative thallus resembles the algal thallus in general construction.

8.The hyphal walls off oomycetes contain aminoacid hydroxyprotine which is not found other fungi, but is characteristic of the cell walls of green algae.

9. A number of biochemical synthetic pathways in the oomycetes are very different than those present on all other true fungi. Oomycetes like the plants synthesise lysine using diaminopimelic acid pathway rather than through the aminoadipic acid pathway.

10. Oomycetes have tubular mitochondrial cristae. All other fungi have plate like mitochondrial cristae.

11.Sexual reproduction in oomycetes in oogaamous. It takes place by gametagial contact resulting in a characteristic thick walled resting spore, called an oospore.

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Difference between Chromosomal DNA and Plasmid DNA (Chromosomal DNA vs Plasmid DNA)

Difference between Chromosomal DNA and Plasmid DNA (Chromosomal DNA vs Plasmid DNA)

Chromosomal DNA contains instructions as genes for survival, growth and reproduction of an organism. Plasmids are extra chromosomal, self-replicating DNA present in prokaryotes.

Chromosomal DNA vs Plasmid DNA

Chromosomal DNA	Plasmid DNA
Chromosomal DNA carries genetic information as genes in all organisms except some viruses	Plasmids are double stranded circular DNA present in prokaryotes
Chromosomal DNA is genomic DNA	Plasmid DNA is extra chromosomal DNA
Chromosomal DNA present in both prokaryotes and eukaryotes	Plasmid DNA is present only in prokaryotes
Chromosomal DNA is linear in eukaryotes and circular in prokaryotes	Plasmid DNA is always circular in prokaryotes
Chromosomal DNA much larger in size than plasmid DNA	Plsamid DNA size up to 200kb
Generally 1 copy of chromosomal DNA in haploid organisms and 2 copies in diploid organisms	Plasmid copy number per cell ranges from 15 to 700 copies per cell. 15/cell called as low copy number plasmids and 700/cell as high copy number plasmids
Chromosomal DNA replicates only with the replication of genomic DNA	Plasmid DNA can replicate independent of bacterial genomic DNA replication
Chromosomal DNA in eukaryotes have exons and introns, prokaryotic chromosomal DNA has open reading frame, that is with only coding sequences	Plasmid has open reading frame, that is devoid of introns or non-coding sequences
Chromosomal DNA carries genes essential for the survival of organism	Plasmid DNA generally contain genes that codes for proteins involved in antibiotic resistance (R plasmid), fertility factor (F plasmids, or colicin production (Col plasmid)
Chromosmal DNA is used in the study of genome of an organisms	Plasmids are widely used in rDNA technology as cloning vector

*Colicin: Colicins are bactericidal proteins produced by some strains of bacteria, that kills bacteria of the same species.

Chromosomal DNA contains instructions as genes for survival, growth and reproduction of an organism. Plasmids are extra chromosomal, self-replicating DNA present in prokaryotes.

Chromosomal DNA vs Plasmid DNA

Chromosomal DNA	Plasmid DNA
Chromosomal DNA carries genetic information as genes in all organisms except some viruses	Plasmids are double stranded circular DNA present in prokaryotes
Chromosomal DNA is genomic DNA	Plasmid DNA is extra chromosomal DNA
Chromosomal DNA present in both prokaryotes and eukaryotes	Plasmid DNA is present only in prokaryotes
Chromosomal DNA is linear in eukaryotes and circular in prokaryotes	Plasmid DNA is always circular in prokaryotes
Chromosomal DNA much larger in size than plasmid DNA	Plsamid DNA size up to 200kb
Generally 1 copy of chromosomal DNA in haploid organisms and 2 copies in diploid organisms	Plasmid copy number per cell ranges from 15 to 700 copies per cell. 15/cell called as low copy number plasmids and 700/cell as high copy number plasmids
Chromosomal DNA replicates only with the replication of genomic DNA	Plasmid DNA can replicate independent of bacterial genomic DNA replication
Chromosomal DNA in eukaryotes have exons and introns, prokaryotic chromosomal DNA has open reading frame, that is with only coding sequences	Plasmid has open reading frame, that is devoid of introns or non-coding sequences
Chromosomal DNA carries genes essential for the survival of organism	Plasmid DNA generally contain genes that codes for proteins involved in antibiotic resistance (R plasmid), fertility factor (F plasmids, or colicin production (Col plasmid)
Chromosmal DNA is used in the study of genome of an organisms	Plasmids are widely used in rDNA technology as cloning vector

*Colicin: Colicins are bactericidal proteins produced by some strains of bacteria, that kills bacteria of the same species.

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Difference between Mass Selection and Pureline Selection (Mass Selection vs Pureline Selection)

Difference between Mass Selection and Pureline Selection (Mass Selection vs Pureline Selection)

Selection is the oldest breeding method. This is the basis for all crop improvement methods. Selection is of two types viz., natural selection and artificial selection. Artificial selection is of three types: mass selection, pureline selection and clonal selection.

Mass selection: This is the simplest and the oldest method  of crop improvement practiced by farmers. It is practiced in crop pollinated crops.

Pureline selction: The term pureline was first introduced by W. L. Johannsen of Denmark 1903. A pureline may be defined as the progeny of a single individual obtained by selfing”.

Mass selection vs Pureline Selection

Mass Selection

1. Used in both cross and self pollinated species.

2. Genetic variation is present.

3. Mass selected variety has wide adaptation

4. Produce of variety is less uniform.

Pureline Selection

1. Used in self pollinated species

2. Genetic variation is absent.

3. Pure line variety has narrow adaptation

4. Produce of variety is highly uniform

Selection is the oldest breeding method. This is the basis for all crop improvement methods. Selection is of two types viz., natural selection and artificial selection. Artificial selection is of three types: mass selection, pureline selection and clonal selection.

Mass selection: This is the simplest and the oldest method  of crop improvement practiced by farmers. It is practiced in crop pollinated crops.

Pureline selction: The term pureline was first introduced by W. L. Johannsen of Denmark 1903. A pureline may be defined as the progeny of a single individual obtained by selfing”.

Mass selection vs Pureline Selection

Mass Selection

1. Used in both cross and self pollinated species.

2. Genetic variation is present.

3. Mass selected variety has wide adaptation

4. Produce of variety is less uniform.

Pureline Selection

1. Used in self pollinated species

2. Genetic variation is absent.

3. Pure line variety has narrow adaptation

4. Produce of variety is highly uniform

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7 Differences between DNA and DNase (DNA vs DNase)

7 Differences between DNA and DNase (DNA vs DNase)

DNA (Deoxyribonucleic acid) is the genetic material made up nucleotides whereas DNase (Deoxyribonucleases) is the enzyme that catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA. It non-specifically cleaves DNA to release 5'-phosphorylated di-, tri-, and oligonucleotide products. The relationship between DNA and DNase is that the DNA is the substrate for the enzyme DNase.

DNA vs DNAase

DNA	DNase
Chemically DNA is a nucleic acid	DNase is a protein
The building blocks or monomer of DNA is nucleotides	As DNase is a protein, it is made up of amino acids
Function: Self replicating genetic material and major constituents of chromosomes. Hereditary material in all organisms except some few viruses	Function: Deoxyribonucleases are one type of nuclease, capable of hydrolyzing phosphodiester bonds that link nucleotides of DNA
DNA is found in nucleus	DNase is primarily found in cytoplasm
New DNA strands are synthesized by DNA replication	DNase enzyme is synthesized by the transcription and translation of DNase gene
DNA serves as genetic material for all organisms except for few viruses	It is used to break the double stranded DNA molecule or single stranded DNA molecule into component nucleotides.
DNA contain instructions as genes for synthesis of proteins required for cellular activities	Major Applications -1. Degradation of contaminating DNA after RNA isolation, 2"Clean-up" of RNA prior to RT-PCR and after in vitro transcription, 3. Identification of protein binding sequences on DNA (DNase I footprinting)

DNA (Deoxyribonucleic acid) is the genetic material made up nucleotides whereas DNase (Deoxyribonucleases) is the enzyme that catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA. It non-specifically cleaves DNA to release 5'-phosphorylated di-, tri-, and oligonucleotide products. The relationship between DNA and DNase is that the DNA is the substrate for the enzyme DNase.

DNA vs DNAase

DNA	DNase
Chemically DNA is a nucleic acid	DNase is a protein
The building blocks or monomer of DNA is nucleotides	As DNase is a protein, it is made up of amino acids
Function: Self replicating genetic material and major constituents of chromosomes. Hereditary material in all organisms except some few viruses	Function: Deoxyribonucleases are one type of nuclease, capable of hydrolyzing phosphodiester bonds that link nucleotides of DNA
DNA is found in nucleus	DNase is primarily found in cytoplasm
New DNA strands are synthesized by DNA replication	DNase enzyme is synthesized by the transcription and translation of DNase gene
DNA serves as genetic material for all organisms except for few viruses	It is used to break the double stranded DNA molecule or single stranded DNA molecule into component nucleotides.
DNA contain instructions as genes for synthesis of proteins required for cellular activities	Major Applications -1. Degradation of contaminating DNA after RNA isolation, 2"Clean-up" of RNA prior to RT-PCR and after in vitro transcription, 3. Identification of protein binding sequences on DNA (DNase I footprinting)

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