Sothern blotting
Northern blotting
- The technique was developed by E.M. Southern in 1975.
- The Southern blot is used to detect the presence of a particular DNA fragment in a sample.
- The DNA detected can be a single gene, or it can be part of a larger piece of DNA such as a viral genome.

- The technique was developed by Alwine and his colleagues in 1979
- Technique for detecting specific RNAs separated by electrophoresis by hybridization to a labeled DNA probe.

Southern blotting
|
Northern blotting
|
Southern name of inventor | Northern a misnomer |
Separation of DNA | Separation of RNA |
Denaturation needed | Denaturation not needed |
Nitrocellulose filter membrane | Amino benzyloxymethyl filter paper Membrane |
DNA-DNA Hybridization | RNA-DNA Hybridization |