Difference between Southern and Northern Blotting

Sothern blotting
  • The technique was developed by E.M. Southern in 1975.
  • The Southern blot is used to detect the presence of a particular DNA fragment in a sample.
  • The DNA detected can be a single gene, or it can be part of a larger piece of DNA such as a viral genome.
Southern blotting Procedure Northern blotting
  • The technique was developed by Alwine and his colleagues in 1979
  • Technique for detecting specific RNAs separated by electrophoresis by hybridization to a labeled DNA probe.
Northern Blotting Procedure
Southern blotting
Northern blotting
Southern name of inventor Northern a misnomer
Separation of DNA Separation of RNA
Denaturation needed Denaturation  not needed
Nitrocellulose filter membrane Amino benzyloxymethyl filter paper Membrane
DNA-DNA  Hybridization RNA-DNA Hybridization
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