7 Differences between DNA and DNase (DNA vs DNase)

DNA (Deoxyribonucleic acid) is the genetic material made up nucleotides whereas DNase (Deoxyribonucleases) is the enzyme that catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA. It non-specifically cleaves DNA to release 5'-phosphorylated di-, tri-, and oligonucleotide products. The relationship between DNA and DNase is that the DNA is the substrate for the enzyme DNase.

DNA vs DNAase

DNA	DNase
Chemically DNA is a nucleic acid	DNase is a protein
The building blocks or monomer of DNA is nucleotides	As DNase is a protein, it is made up of amino acids
Function: Self replicating genetic material and major constituents of chromosomes. Hereditary material in all organisms except some few viruses	Function: Deoxyribonucleases are one type of nuclease, capable of hydrolyzing phosphodiester bonds that link nucleotides of DNA
DNA is found in nucleus	DNase is primarily found in cytoplasm
New DNA strands are synthesized by DNA replication	DNase enzyme is synthesized by the transcription and translation of DNase gene
DNA serves as genetic material for all organisms except for few viruses	It is used to break the double stranded DNA molecule or single stranded DNA molecule into component nucleotides.
DNA contain instructions as genes for synthesis of proteins required for cellular activities	Major Applications -1. Degradation of contaminating DNA after RNA isolation, 2"Clean-up" of RNA prior to RT-PCR and after in vitro transcription, 3. Identification of protein binding sequences on DNA (DNase I footprinting)