Genomics vs Metagenomics Similarities and differences
Both fields involve DNA sequencing but differ
in scale and dimension, genomics focuses on individual organisms, while
metagenomics explores whole communities present in an environmental science.
Genomics |
Metagenomics |
Study of an
individual organism’s genome. |
Study of the
complete set of genomes in a sample (bacteria, viruses, fungi, protozoa etc.) |
Focus is on single
organism’s genetic makeup. |
Collection of
genomes from multiple individuals within an environment or clinical sample. |
Sequencing a
specific species’ genome. |
Analyzing
genomes directly from a mixed sample without culturing. |
Methodology
involves Genomic DNA Isolation, Separation of DNA,
Cutting and Joining of DNA: Cloning in Vectors: Detection of Gene of
Interest: making of Recombinant DNA, DNA amplification (by PCR) and DNA
sequencing |
Broad sampling
by direct sequencing without relying on cultured cells. Sample collection and
DNA extraction, DNA sequencing, assembly, binning*, gene prediction and
annotation |
Applications:
|
|
Example: Human Genome Project (Sequencing the human genome) Chimpanzee
genome project E.coli genome project Arabidopsis
genome project |
Analyzing
microbial communities in soil, sea, fresh water, pond etc. (Environmental samples) or skin, gut microbiome (clinical samples) |
*Binning*:
Group sequences based on similarity to identify different microbial species or
functional groups.
Post a Comment
We Love to hear from U :) Leave us a Comment to improve this site
Thanks for Visiting.....