Difference between RAPD and RFLP

RAPD ( Random Amplified Polymorphic DNA)

Definition: If primers with arbitrary sequences are used for amplification, DNA segments to be amplified will be selected at random and this provides truly random sample of DNA markers and so is described as random amplified polymorphic DNA (RAPD).

RFLP : Restriction Fragment Length Polymorphism
RAPD

RAPD ( Random Amplified Polymorphic DNA)

1. Quantity of DNA required for analysis is small (10-50 ng)

2. Same primers with arbitrary sequence can be used for different species

3. Fewer steps in procedure therefore it is rapid (Five times quicker than RFLP)

4. Technique comparatively less reliable

5. Cannot detect allelic variants

6. 1-10 loci detected

7. Method involves:
       a) extraction of DNA,
       b) amplification by PCR using random primers,
       c) gel electrophoresis of amplified DNA and visualisation of markers

RFLP (Restriction Fragment Length Polymorphism)
RFLP

1. Large quantity of purified DNA required i.e. 2-10 ug

2. Different species specific probes are required.

3. Comparatively slower processing due to more steps involved.

4. Technique comparatively more reliable

5. Can detect allelic variants

6. 1-3 loci detected

7. Method involves:
       a) digestion of extracted DNA by restriction enzymes,
       b) gel electrophoresis of fragments,
       c) southern blot by specific probes and detection of specific sequences
Sharing is Caring ..... Please take 5 seconds to Share. Thank you...

1 comments:

Post a Comment

We Love to hear from U :) Leave us a Comment.

Your Comments will Definitely Make a Difference in Improving this Site

Thanks for Visiting.....

Related Posts Plugin for WordPress, Blogger...

Follow by Email

 
2014 Major Differences | MajorDifferences.com. Our Partners Biology Exams 4 U, Biology Quizzes, MCQ Biology