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Difference between Lock and Key hypothesis and Induced fit hypothesis

There are two views regarding the mode of action of enzymes: Lock and Key hypothesis (theory)
Induced fit hypothesis (theory).
Lock and Key Theory: Emil Fisher proposed this hypothesis in 1894. According to this hypothesis the active site of the enzyme is like a ‘lock’ into which substrate fits like a ‘key’.i.e., the shape of the active site and the substrate molecules are complementary . So the enzyme molecule holds the substrate, molecule close together, forming the unusable intermediate compound, the enzyme substrate complex. It dissociates to form enzyme and products.
lock and key
Induced Fit Theory: Daniel E.Koshland formulated this hypothesis in 1959. According to this hypothesis the active site does not have a rigid ‘lock and key’ conformation. The binding of the substrate molecule to the enzyme molecule induces to modify the shape of the active site so that it becomes complementary to the substrate molecule. This is called the induced fit . Induced fit is possible because of the flexibility of the protein molecules.

Lock and Key Theory vs Induced fit Theory
Lock and Key Theory:
1. Active site is a single entity.
2. There is no separate catalytic group.
3. Active site is static.
4. Development of transition state is not considered.
5.It does not visualize the weakening of substrate bonds.
6. It does not explain the mechanism of non activity in case of competitive inhibitor.
Induced fit Theory:
1. Active  site is made of two components.
2. A separate catalytic group is visualized.
3. Active site is not static.
4. It considers the development of transition state before the reacants undergo change.
5. Catalytic group is believed to weaken the substrate bonds by nucleophilic and electrophilic attack.
6. It explains the a mechanism for nonaction over competitive inhibitor.


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